Protein and Lactose Separation by Modified Ultrafiltration Membrane using Layer by Layer Technique

Layer-by-layer Cell Membrane Assembly

Eukaryotic subcellular membrane systems, such as the nuclear envelope or endoplasmic reticulum, present a rich array of architecturally and compositionally complex supramolecular targets that are as yet inaccessible. Here we describe layer-by-layer phospholipid membrane assembly on microfluidic droplets, a route to structures with defined compositional asymmetry and lamellarity. Starting with p...

Mechanically durable, superoleophobic coatings prepared by layer-by-layer technique for anti-smudge and oil-water separation

Superoleophobic surfaces are of interest for anti-fouling, self-cleaning, anti-smudge, low-drag, anti-fog, and oil-water separation applications. Current bioinspired surfaces are of limited use due to a lack of mechanical durability. A so-called layer-by-layer approach, involving charged species with electrostatic interactions between layers, can provide the flexibility needed to improve adhesi...

Transport phenomena in ultrafiltration: membrane selectivity and boundary layer phenomena

Retention characteristics of UF membranes are de— lineated through comparison of the combined viscous flow and frictional model with the Ferry—Faxen equation. The molecular weight cut—off concept is discussed using calculations of how sharp cut-off curves one can expect of UF membranes with uniform pore sizes and how heteroporosity will affect such curves. Experimental retention—flux curves for...

Preparation of Gnathostoma protein by ultrafiltration method using Nanosep membrane.

We report our experience with Gnathostoma protein preparation by the ultrafiltration method. Crude antigen was sonicated and ultrafiltrated using the Nanosep 100 K membrane. SDS-PAGE electrophoresis showed protein bands at 43, 41, 24, 22, 21, 19.5 kDa. Use of the ultrafiltration method can provide specific protein (24 kDa), similar to the non-ultrafiltration method, with the other 5 non-specifi...

Antitumoral materials with regenerative function obtained using a layer-by-layer technique

A layer-by layer technique was successfully used to obtain collagen/hydroxyapatite-magnetite-cisplatin (COLL/HAn-Fe3O4-CisPt, n=1-7) composite materials with a variable content of hydroxyapatite intended for use in the treatment of bone cancer. The main advantages of this system are the possibility of controlling the rate of delivery of cytostatic agents, the presence of collagen and hydroxyapa...

Template Assisted Layer-by-layer Fabrication of Protein Nanotubes